Induction of defence gene expression

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Induction of defence gene expression by oligogalacturonic acid requires increases in both cytosolic calcium and hydrogen

Abstract: Responses to oligogalacturonic acid (OGA) were determined in transgenic Arabidopsis thaliana seedlings expressing the calcium reporter protein aequorin. OGA stimulated a rapid, substantial and transient increase in the concentration of cytosolic calcium ([Ca2+]cyt) that peaked after ca. 15 s. This increase was dose-dependent, saturating at ca. 50 μg Gal equiv/ml of OGA. OGA also stimulated a rapid generation of H2O2. A small, rapid increase in H2O2 content was followed by a much larger oxidative burst, with H2O2 content peaking after ca. 60 min and declining thereafter. Induction of the oxidative burst by OGA was also dose-dependent, with a maximum response again being achieved at ca. 50 μg Gal equiv/mL. Inhibitors of calcium fluxes inhibited both increases in [Ca2+]cyt and [H2O2], whereas inhibitors of NADPH oxidase blocked only the oxidative burst. OGA increased strongly the expression of the defence-related genes CHS,GST, PAL and PR-1. This induction was suppressed by inhibitors of calcium flux or NADPH oxidase, indicating that increases in both cytosolic calcium and H2O2 are required for OGA-induced gene expression. 作 者: Xiang Yang HU    Steven J NEILL    Wei Ming CAI    Zhang Cheng TANG   作者单位: Xiang Yang HU,Wei Ming CAI,Zhang Cheng TANG(Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China)

Steven J NEILL(Centre for Research in Plant Science, University of the West of England, Bristol, Coldharbour Lane, Bristol BS16 1QY, UK) 

期 刊: 细胞研究(英文版)  ISTICSCI   Journal: CELL RESEARCH  年,卷(期): 2004, 14(3)  分类号:   Keywords: Arabidopsis thaliana    cytosolic calcium    defence gene expression    hydrogen peroxide    OGA