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Effects of Formaldehyde Inhalation on Lung of Rats
Objective To analyze protein changes in the lung of Wistar rats exposed to gaseous formaldehyde (FA) at 32-37 mg/m3 for 4 h/day for 15 days using proteomics technique. Methods Lung samples were solubilized and separated by two-dimensional electrophoresis (2-DE), and gel patterns were scanned and analyzed for detection of differently expressed protein spots. These protein spots were identified by MALDI-TOF-MS and NCBInr protein database searching. Results Four proteins were altered significantly in 32-37 mg/m3 FA group, with 3 proteins up-regulated, 1 protein down-regulated. The 4 proteins were identified as aldose reductase, LIM protein, glyceraldehyde-3-phosphate dehydrogenase, and chloride intracellular channel 3. Conclusion The four proteins are related to cell proliferation induced by FA and defense reaction of anti-oxidation. Proteomics is a powerful tool in research of environmental health, and has prospects in search for protein markers for disease diagnosis and monitoring.
作 者： YU-HUA YANG ZHU-GE XI FU-HUAN CHAO DAN-FENG YANG 作者单位： Institute of Health and Environmental Medicine, Tianjin 300050, China 刊 名： 生物医学与环境科学（英文版） SCI 英文刊名： BIOMEDICAL AND ENVIRONMENTAL SCIENCES 年，卷(期)： 2005 18(3) 分类号： X3 关键词： Proteomics Formaldehyde Rat Lung Toxicity Two-dimensional electrophoresis Mass spectrometry