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纳米银抗菌活性和细胞毒性评价
摘要:以大肠杆菌和金黄色葡萄球菌做模型检测抗菌活性,选择L-929细胞通过MTT法评价毒性.结果证明纳米银具有显著的抗菌活性且呈剂量依赖性;不论在液体培养基还是固体培养基,30×10浓度产生的抗菌作用大于50%,但有潜在的细胞毒性;即使在3×10浓度下.细胞毒性达到2级,有26%,~50%的细胞生长受到抑制.Abstract:E. coil and S. aureus were used as model for testing antimicrobial activity, and L-929 cell line was selected for toxicity evaluation by cellular mitochondrial function(MTT assay). The results show that silver nanoparticles(Ag NPs)can significantly in-crease antibacterial activity with the increasing of concentration of the silver nanoparticles. The lowest effective concentration of silver nanoparticles(30 X 10)which leads to more than 50% of inhibition in liquid medium and agar plate on bacterias is still satis-factory but will produce potential cytotoxicity. Even at 3 x 10 concentration of silver nanoparticles the cytotoxicity still exists with 2grade which represents 26%-50% of cell growth inhibited, and 30×10 displays similar impact. 作者: 李新平[1]李胜利[2]张淼涛[1]张文龙[1]李创宏[1] Author: Li Xinping[1] Li Shengli[2] Zhang Miaotao[1] Zhang Wenlong[1] Li Chuanghong[1] 作者单位: 西北农林科技大学,陕西,杨凌,712100中国农业大学动物营养国家重点实验室,北京,100083 期 刊: 稀有金属材料与工程 ISTICEISCIPKU Journal: RARE METAL MATERIALS AND ENGINEERING 年,卷(期): 2011, 40(2) 分类号: Q2 关键词: 纳米银 大肠杆菌 金黄色葡萄球菌 抗菌活性 细胞毒性 Keywords: silver nanoparticles S. aureus E. coli antibacterial activity cytotoxicity 机标分类号: O6 P61 机标关键词: 纳米银 抗菌活性 细胞毒性评价 Ag Nanoparticles silver nanoparticles antimicrobial activity antibacterial activity toxicity evaluation 金黄色葡萄球菌 cell growth 液体培养基 剂量依赖性 固体培养基 L-929细胞 effective cell line 细胞生长 浓度 模型检测 抗菌作用 基金项目: National Natural Science Foundation of China【纳米银抗菌活性和细胞毒性评价】相关文章:
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