- 相关推荐
Induction of animal model of Graves disease in BALB/c mice
Objective To construct an animal model of Graves' disease (GD) by immunizing BALB/c mice with hM12 cells co-expressing major histocompatibility complex (MHC) class Ⅱ molecules and human thyrotropin receptor (TSHR) molecules. Methods BALB/c mice in experimental group (H-2d) were immunized with hM12 cells Intraper-itoncally every 2 weeks for six times, while mice in control group were immunized with M12 cells. Five weeks later, the thyroids were histologically examined, and serum samples were tested for thyroid-stimulating antibodies (TSAb) and thyroid hormone levels. Results One BALB/c mouse in experimental group developed Graves'-like disease. Total T4 and T3 levels in this mouse were above the upper limit of normal, TSAb activity was displayed in its serum. The thyroid histologically showed the features of thyroid hyperactivity including thyrocyte hypercellularity and colloid absorption.None of control mice developed Graves'-like disease. Conclusion An animal model with some characteristics of human Graves' disease was successfully induced and the model will facilitate studies aimed directly at understanding the patho-genesis of autoimmunity in Graves' disease.
作 者： Zhu-fang Tian Bing-yin Shi Xiao-yan Wu Li Xu 作者单位： Zhu-fang Tian(Department of Endocrinology, the First Affiliated Hospital, Medical School of Xi'an Jiaotong Uni-versity, Xi'an 710061;Department of Endocrinology, Xi'an Central Hospital, Xi'an 710003, China)Bing-yin Shi,Xiao-yan Wu,Li Xu(Department of Endocrinology, the First Affiliated Hospital, Medical School of Xi'an Jiaotong Uni-versity, Xi'an 710061)
刊 名： 西安交通大学学报（英文版） 英文刊名： ACADEMIC JOURNAL OF XI'AN JIAOTONG UNIVERSITY(ENGLISH EDITION) 年，卷(期)： 2009 21(4) 分类号： Q786 R347.514 关键词： major histocompatibility complex Ⅱ thyrotropin receptor M12 cell Graves' disease【Induction of animal model of Graves 】相关文章：
Pelagic Nitrogen Cycling in Jiaozhou Bay, a Model Study I: the Conceptual Model04-29
Multiresolution Terrian Model in GIS04-28
无线model怎么设置01-01
Lattice Model with Traveling Compactons04-29
Improving the numerical stability of the MAGIC model04-28
Theoretical model and quantification of reflectance photometer04-28